2B0Q
Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Neomycin B Complex
Replaces: 1L8UExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 46.357, 46.357, 301.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.360 - 2.700 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l8t |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.396 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.048 | 0.168 |
| Number of reflections | 8074 | |
| <I/σ(I)> | 11.3 | 4.3 |
| Completeness [%] | 80.8 | |
| Redundancy | 3.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 277 | PEG 600, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
