2B0O
Crystal structure of UPLC1 GAP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.055, 106.716, 100.365 |
Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.060 |
R-factor | 0.18518 |
Rwork | 0.183 |
R-free | 0.23264 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dcq |
RMSD bond length | 0.019 |
RMSD bond angle | 1.772 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.059 | 2.059 |
Number of reflections | 67919 | |
<I/σ(I)> | 17.2 | 2.4 |
Completeness [%] | 97.6 | 88.8 |
Redundancy | 4.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | NaCitrat 1.5M, Ethylene Glycol 9%, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |