2B0O
Crystal structure of UPLC1 GAP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.055, 106.716, 100.365 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.060 |
| R-factor | 0.18518 |
| Rwork | 0.183 |
| R-free | 0.23264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dcq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.772 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.059 | 2.059 |
| Number of reflections | 67919 | |
| <I/σ(I)> | 17.2 | 2.4 |
| Completeness [%] | 97.6 | 88.8 |
| Redundancy | 4.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | NaCitrat 1.5M, Ethylene Glycol 9%, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
