2B0A
Crystal structure of protein MJ0783 from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 87 |
| Detector technology | CCD |
| Collection date | 2005-09-02 |
| Detector | ADSC QUANTAM Q315 |
| Wavelength(s) | 0.989 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 67.942, 73.156, 46.570 |
| Unit cell angles | 90.00, 122.87, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.450 |
| R-factor | 0.18608 |
| Rwork | 0.185 |
| R-free | 0.21823 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.392 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.481 |
| Number of reflections | 34059 | |
| <I/σ(I)> | 16.1 | 2.1 |
| Completeness [%] | 98.0 | 96.7 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 30% PEG 550, 100 mM Tris-HCl, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
