2B00
Crystal Structure of Porcine Pancreatic Phospholipase A2 in Complex with Glycocholate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2004-12-14 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 69.577, 69.577, 64.184 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.270 - 1.850 |
Rwork | 0.186 |
R-free | 0.21000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.073 | 0.548 |
Number of reflections | 15741 | |
<I/σ(I)> | 20 | 2.6 |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.4 M Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |