2AZW
Crystal structure of the MutT/nudix family protein from Enterococcus faecalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 90.839, 90.839, 52.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.280 - 1.900 |
R-factor | 0.19479 |
Rwork | 0.193 |
R-free | 0.22969 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.097 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.280 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.086 | 0.662 |
Number of reflections | 17228 | |
<I/σ(I)> | 40.2 | 2.7 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 9.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |