2AZW
Crystal structure of the MutT/nudix family protein from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.839, 90.839, 52.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.280 - 1.900 |
| R-factor | 0.19479 |
| Rwork | 0.193 |
| R-free | 0.22969 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.097 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.280 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.086 | 0.662 |
| Number of reflections | 17228 | |
| <I/σ(I)> | 40.2 | 2.7 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 9.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1M HEPES, 25% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
