2AZO

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Experimental procedure

Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	39.860, 111.570, 54.930
Unit cell angles	90.00, 96.53, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.225 *
Rwork	0.202
R-free	0.28900 *
Structure solution method	SIRAS
RMSD bond length	0.006 *
RMSD bond angle	1.399 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.370
High resolution limit [Å]	2.300	2.280
Rmerge	0.079	0.369
Number of reflections	20757
<I/σ(I)>	10	2
Completeness [%]	95.2	83.8
Redundancy	2.6	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8 *	20 *	100MM AMMONIUM ACETATE, 50 MM MAGNESIUM ACETATE, 1 MM DTT AND 12-16 % PEG6000, pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium acetate	100 (mM)
2	1	reservoir	magnesium acetate	50 (mM)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	PEG6000	12-16 (%)