2AZL
Crystal structure for the mutant F117E of Thermotoga maritima octaprenyl pyrophosphate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 151.514, 151.514, 64.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.230 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1v4e |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 9443 | |
| <I/σ(I)> | 27.5 | 4.2 |
| Completeness [%] | 99.0 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na+HEPES, Li2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
