2AZK

Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2005-05-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	92.529, 92.529, 127.183
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.350 - 2.700
R-factor	0.247
Rwork	0.247
R-free	0.32000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2azj
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	17750
<I/σ(I)>	17.9	3.1
Completeness [%]	99.8	99.9
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	PEG 6000, lithium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K