2AZJ
Crystal structure for the mutant D81C of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-12-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 91.926, 91.926, 121.810 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.370 - 2.400 |
R-factor | 0.317 |
Rwork | 0.317 |
R-free | 0.34700 |
Structure solution method | MIR |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 23556 | |
<I/σ(I)> | 27.4 | 3.7 |
Completeness [%] | 98.7 | 98.6 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 6000, lithium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |