2AZC
HIV-1 Protease NL4-3 6X mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-19 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 100.242, 100.242, 97.119 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.880 - 2.010 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.27800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2az8 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.830 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.880 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.050 | 0.378 |
Number of reflections | 16751 | |
<I/σ(I)> | 14.4 | 3.9 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 4.84 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.2 | 297.16 | ammonium sulfate, sodium acetate, sodium thiocyanate, VAPOR DIFFUSION, SITTING DROP, temperature 297.16K, pH 5.20 |