2AZ0
Flock House virus B2-dsRNA Complex (P212121)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9190 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.261, 86.232, 94.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.400 - 2.600 |
| R-factor | 0.242 |
| Rwork | 0.241 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.400 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 13519 | |
| Completeness [%] | 98.9 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 3350, magnesium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
