2AZ0

Flock House virus B2-dsRNA Complex (P212121)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	93
Detector technology	CCD
Collection date	2005-07-15
Detector	MARRESEARCH
Wavelength(s)	0.9190
Spacegroup name	P 21 21 21
Unit cell lengths	50.261, 86.232, 94.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.400 - 2.600
R-factor	0.242
Rwork	0.241
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.690
High resolution limit [Å]	2.550	2.550
Number of reflections	13519
Completeness [%]	98.9	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	PEG 3350, magnesium sulfate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K