2AYN
Structure of USP14, a proteasome-associated deubiquitinating enzyme
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1, 0.9793, 0.9795, 0.95 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.285, 121.582, 166.852 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 - 3.200 |
R-factor | 0.262 |
Rwork | 0.261 |
R-free | 0.32200 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 27320 | |
Completeness [%] | 98.9 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 290 | MES, ammonium sulfate, PEG monomethyl ether, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |