2AYG
Crystal structure of HPV6a E2 DNA binding domain bound to an 18 base pair DNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 61 |
| Unit cell lengths | 73.449, 73.449, 109.237 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.630 - 3.100 |
| R-factor | 0.247 |
| Rwork | 0.203 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.630 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.118 | 0.351 |
| Number of reflections | 6087 | |
| <I/σ(I)> | 17.9 | 4.2 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | sodium chloride, Hepes, ammonium sulphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium chloride | ||
| 2 | 1 | 1 | Hepes | ||
| 3 | 1 | 1 | ammonium sulphate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | sodium chloride | ||
| 6 | 1 | 2 | Hepes | ||
| 7 | 1 | 2 | ammonium sulphate | ||
| 8 | 1 | 2 | H2O |
