2AYD
Crystal Structure of the C-terminal WRKY domainof AtWRKY1, an SA-induced and partially NPR1-dependent transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.24 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 45.552, 45.552, 68.962 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.20400 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.606 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | 0.354 |
| Number of reflections | 11069 | |
| Completeness [%] | 97.9 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | succinic acid, Tris HCl, PEG MME 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
