2AXQ

Apo histidine-tagged saccharopine dehydrogenase (L-Glu forming) from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-08-18
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	85.279, 85.279, 141.977
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.850 - 1.700
R-factor	0.2
Rwork	0.198
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e5l used monomer backbone only
RMSD bond length	0.014
RMSD bond angle	1.407
Data reduction software	CrystalClear (D*TREK (MSC/RIGAKU))
Data scaling software	d*TREK (9.2D)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.850	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.056	0.368
Number of reflections	64775
<I/σ(I)>	15.8	4.3
Completeness [%]	97.6	95.9
Redundancy	7.18	4.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	1.2M Ammonium sulfate, 25mM Bis-Tris, 6.5-7mg/mL enzyme, 50mM Tris-HCl pH 8.0, 150mM KCl, 75mM imidazole, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K