2AXP
X-Ray Crystal Structure of Protein BSU20280 from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR256.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97575 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.847, 99.944, 78.344 |
Unit cell angles | 90.00, 125.29, 90.00 |
Refinement procedure
Resolution | 29.780 - 2.500 |
R-factor | 0.255 |
Rwork | 0.255 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.074 | 0.333 |
Number of reflections | 38099 | |
<I/σ(I)> | 15 | 3.2 |
Completeness [%] | 99.2 | 95.8 |
Redundancy | 4 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate (pH 6.5), 17% PEG 8k, 240 mM magnesium acetate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 293K |