2AWI
Structure of PrgX Y153C mutant
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-03 |
| Wavelength(s) | 0.97926, 0.97945, 0.97772 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.527, 134.366, 195.199 |
| Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.250 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.27100 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.900 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 212720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5 | 298 | PEG 4000, citrate-phophate, pH 5.0, VAPOR DIFFUSION, temperature 298K |
