2AWH
Human Nuclear Receptor-Ligand Complex 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 65 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939261 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.220, 96.717, 92.304 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 43.030 - 2.000 |
| R-factor | 0.238 |
| Rwork | 0.212 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (CCP4_3.1.9) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Outer shell | |
| Low resolution limit [Å] | 2.110 |
| High resolution limit [Å] | 2.000 |
| Total number of observations | 25088 |
| Completeness [%] | 96.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 5% PEG 8000, 200mM KCl, 40mM bis-tris-propane pH 8.5, 2.5% 1,2-propanediol, 0.5% n-heptyl-B-D-glucopyranoside, 1mM EDTA, 10mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
