2AWF
Structure of human Ubiquitin-conjugating enzyme E2 G1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97985 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 59.444, 59.444, 186.812 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.980 - 2.100 |
R-factor | 0.22289 |
Rwork | 0.221 |
R-free | 0.26337 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pzv |
RMSD bond length | 0.015 |
RMSD bond angle | 1.570 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.700 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.037 | 0.647 |
Number of reflections | 13412 | |
<I/σ(I)> | 72.07 | 2.25 |
Completeness [%] | 96.4 | 84.6 |
Redundancy | 15.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 24% PEG3350, 0.2 M MgAc, 0.1 M Tris, pH 7.5, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |