2AW9
Superoxide dismutase with manganese from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.5499 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.833, 83.499, 65.495 |
Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
Resolution | 62.990 - 2.700 |
R-factor | 0.1701 |
Rwork | 0.168 |
R-free | 0.21418 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1y67 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.742 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 90.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 11594 | |
<I/σ(I)> | 20.3297 | |
Completeness [%] | 92.6 | 71.7 |
Redundancy | 5.6 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | magnesium chloride, tris-hydrochloride, PEG 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |