2AVU
Structure of the Escherichia coli FlhDC complex, a prokaryotic heteromeric regulator of transcription
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97964 |
Spacegroup name | P 61 |
Unit cell lengths | 151.137, 151.137, 114.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.790 - 3.000 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25500 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.037 | 0.314 |
Number of reflections | 29451 | |
<I/σ(I)> | 28.3 | 4.2 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium chloride, sodium acetate, ethylene imine polymer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | sodium chloride, sodium acetate, ethylene imine polymer, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |