2AVN
CRYSTAL STRUCTURE OF A UBIQUINONE/MENAQUINONE BIOSYNTHESIS METHYLTRANSFERASE-RELATED PROTEIN (TM1389) FROM THERMOTOGA MARITIMA MSB8 AT 2.35 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-04 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97915 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 130.475, 130.475, 192.926 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.800 - 2.350 |
Rwork | 0.169 |
R-free | 0.19900 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.459 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.800 | 29.860 | 2.410 |
High resolution limit [Å] | 2.350 | 10.510 | 2.350 |
Rmerge | 0.183 | 0.066 | 0.981 |
Number of reflections | 40826 | ||
<I/σ(I)> | 3.4 | 8.7 | 0.7 |
Completeness [%] | 99.6 | ||
Redundancy | 9.8 | 10.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5 | 293 | 1.0M LiCl, 10.0% PEG-600, 0.1M Citrate, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 5.0 |
2 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7 | 293 | 40.0% MPD, 0.1M HEPES, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 7.0 |