2AVH
G4T3G4 dimeric quadruplex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2004-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 29.932, 37.112, 43.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.300 - 1.500 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GGGG(+U)TTGGGG chain A (P21) |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | |
| Number of reflections | 4066 | |
| Completeness [%] | 99.0 | 96.127 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 284.15 | sodium iodide, sodium cacodylate, PEG 400, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 284.15K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium iodide | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | sodium iodide | ||
| 6 | 1 | 2 | sodium cacodylate | ||
| 7 | 1 | 2 | PEG 400 | ||
| 8 | 1 | 2 | H2O |
