2AV9
Crystal Structure of the PA5185 protein from Pseudomonas Aeruginosa Strain PAO1.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-05 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97943, 0.97929, 0.96420 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 241.661, 64.384, 117.469 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 36.940 - 2.400 |
| R-factor | 0.20653 |
| Rwork | 0.204 |
| R-free | 0.25334 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.904 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.940 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 | 0.348 |
| Number of reflections | 68671 | |
| <I/σ(I)> | 12.5 | 2.2 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Ammonium Sulphate, 0.1M Bis-Tris pH 5.5, 23% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
