2AUY
Pterocarpus angolensis lectin in complex with the trisaccharide GlcNAc(b1-2)Man(a1-3)Man
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2003-10-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.81 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.800, 83.140, 123.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.950 |
| R-factor | 0.177 |
| Rwork | 0.175 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q8p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.091 | 0.463 |
| Number of reflections | 43030 | |
| <I/σ(I)> | 11.7 | 3.25 |
| Completeness [%] | 97.9 | 92.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Na-cacodylate, Na-acetate, PEG-8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
