2AUY
Pterocarpus angolensis lectin in complex with the trisaccharide GlcNAc(b1-2)Man(a1-3)Man
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.81 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.800, 83.140, 123.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.950 |
R-factor | 0.177 |
Rwork | 0.175 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1q8p |
RMSD bond length | 0.006 |
RMSD bond angle | 1.533 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.000 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.091 | 0.463 |
Number of reflections | 43030 | |
<I/σ(I)> | 11.7 | 3.25 |
Completeness [%] | 97.9 | 92.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Na-cacodylate, Na-acetate, PEG-8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |