2AUA
Structure of BC2332: A Protein of Unknown Function from Bacillus cereus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.222, 76.640, 107.503 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.350 |
R-factor | 0.20632 |
Rwork | 0.204 |
R-free | 0.25388 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.258 |
Data scaling software | SCALEPACK |
Phasing software | ACRS ((HYSS) |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.073 | 0.440 |
Number of reflections | 24016 | |
<I/σ(I)> | 14.3 | 3.3 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M Sodium citrate, 1M Ammonium dihydrogen Phosphate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |