2AU3
Crystal Structure of the Aquifex aeolicus primase (Zinc Binding and RNA Polymerase Domains)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.222, 66.403, 138.157 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.000 |
R-factor | 0.20488 |
Rwork | 0.203 |
R-free | 0.23826 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Low-resolution Aquifex aeolicus DnaG from Tm-soak MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.223 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 25989 | |
<I/σ(I)> | 14.3 | 3.3 |
Completeness [%] | 99.0 | 93.8 |
Redundancy | 3.45 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 291 | 18-22% EtOH, 55 mM HEPES, 100 mM NaCl, pH 7.5, Microbatch, temperature 291K |