2ATV
The crystal structure of human RERG in the GDP bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-05 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 76.405, 76.405, 107.876 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.070 - 1.900 |
R-factor | 0.2056 |
Rwork | 0.204 |
R-free | 0.23350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gua |
RMSD bond length | 0.011 |
RMSD bond angle | 1.380 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 25918 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 277 | PEG 3350, ammonium acetate, Bis Tris buffer , pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K |