2ATS
Dihydrodipicolinate synthase co-crystallised with (S)-lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 121.680, 121.680, 109.799 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.800 - 1.900 |
R-factor | 0.16924 |
Rwork | 0.168 |
R-free | 0.20154 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.395 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.091 | 0.342 |
Number of reflections | 73712 | |
<I/σ(I)> | 3.5 | |
Completeness [%] | 99.4 | 100 |
Redundancy | 3.82 | 3.71 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 284 | 20mM Tris HCl, 10mM (S)-lysine, pH 8, 1.8M K2HPO4, pH 10, N-octyl-R-glucopyranoside, VAPOR DIFFUSION, HANGING DROP, temperature 284K |