2ATR
Acetyltransferase, GNAT family protein SP0256 from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2005-06-14 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9792348 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 71.389, 71.389, 59.179 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.780 - 2.010 |
| R-factor | 0.22107 |
| Rwork | 0.218 |
| R-free | 0.27613 |
| Structure solution method | SAD |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.780 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Number of reflections | 10898 | |
| Completeness [%] | 95.5 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 1.0M (NH4)2HPO4, 0.1M Tris pH 8.5, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
