2ATQ

RB69 single-stranded DNA binding protein-DNA polymerase fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95372
Spacegroup name	P 65
Unit cell lengths	196.029, 196.029, 85.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.990 - 3.200
R-factor	0.321
Rwork	0.284
R-free	0.34500
Structure solution method	MAD
Starting model (for MR)	1ih7 2a1k
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM (5.0)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.068	0.646
Number of reflections	33922
<I/σ(I)>	19.5	1.6
Completeness [%]	99.3	94.9
Redundancy	5.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 400, Tris-Cl, 6-aminocaproic acid, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 400, Tris-Cl, 6-aminocaproic acid, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K