2ATJ
RECOMBINANT HORSERADISH PEROXIDASE COMPLEX WITH BENZHYDROXAMIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1995-03 |
Detector | RIGAKU |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.920, 62.250, 77.990 |
Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
Resolution | 28.600 * - 2.000 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sch |
RMSD bond length | 0.006 |
RMSD bond angle | 1.090 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.085 | 0.209 * |
Total number of observations | 144839 * | |
Number of reflections | 46595 | |
Completeness [%] | 98.5 | 92.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 283 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | BHA | 0.35 (mM) | |
2 | 1 | drop | HRPC | 6.0 (mg/ml) | |
3 | 1 | reservoir | 1.0 (M) | ||
4 | 1 | reservoir | cacodylate | 0.1 (M) |