2AT0
1.00 A Crystal Structure Of L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-20 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 70.286, 42.699, 52.970 |
Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
Resolution | 36.470 - 1.000 |
R-factor | 0.14 |
Rwork | 0.139 |
R-free | 0.15400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1x8o |
RMSD bond length | 0.011 |
RMSD bond angle | 1.489 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.510 | 1.050 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.076 | 0.178 |
Number of reflections | 83901 | |
<I/σ(I)> | 13.9 | 5.3 |
Completeness [%] | 99.8 | 98 |
Redundancy | 4.1 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |