2ASU
Crystal Structure of the beta-chain of HGFl/MSP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.676, 75.848, 47.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.1999 |
| Rwork | 0.198 |
| R-free | 0.23678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bui |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.416 |
| Number of reflections | 23920 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 98.4 | 98.3 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.1 | 292 | 1.4 M NaH2PO4, 0.93 M K2HPO4, CAPS pH 10.5, Li2SO4 0.1 M, pH 6.1, VAPOR DIFFUSION, temperature 292K |
