2ASR

THE THREE-DIMENSIONAL STRUCTURE OF THE ASPARTATE RECEPTOR FROM ESCHERICHIA COLI

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	85.500, 85.500, 103.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.203
Rwork	0.203
RMSD bond length	0.016
RMSD bond angle	2.950
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.300 *
Total number of observations	46244 *
Number of reflections	15309 *
Completeness [%]	86.2 *	65 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	E. coli asparatate receptor	25 (mg/ml)
2	1	drop	ammonium sulfate	200 (mM)
3	1	drop	ammonium sulfate	1.1 (M)	crystallization buffer
4	1	drop	ammonium formate	20 (mM)	crystallization buffer
5	1	drop	formic acid	30 (mM)	crystallization buffer
6	1	reservoir			1 ml of crystallization buffer