2ASN
Crystal structure of D1A mutant of nitrophorin 2 complexed with imidazole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-31 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.389, 34.389, 256.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.300 - 1.700 |
| R-factor | 0.1549 |
| Rwork | 0.152 |
| R-free | 0.20925 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pee |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.782 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.300 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 13482 | |
| <I/σ(I)> | 8.9 | 4.7 |
| Completeness [%] | 73.2 | 38 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
