2ASL
oxoG-modified Postinsertion Binary Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9810 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.218, 101.024, 84.925 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.23792 |
| Rwork | 0.232 |
| R-free | 0.28686 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.456 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.730 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.082 | 0.504 |
| Number of reflections | 34610 | |
| Completeness [%] | 97.5 | 80 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 4000, HEPES-sodium, calcium acetate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | HEPES-sodium | ||
| 3 | 1 | 1 | calcium acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | HEPES-sodium | ||
| 7 | 1 | 2 | calcium acetate | ||
| 8 | 1 | 2 | H2O |
