2ASK
Structure of human Artemin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.631, 33.674, 55.580 |
| Unit cell angles | 90.00, 98.95, 90.00 |
Refinement procedure
| Resolution | 15.600 - 1.550 |
| R-factor | 0.231 |
| Rwork | 0.221 |
| R-free | 0.25500 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.064 | 0.310 |
| Number of reflections | 29225 | |
| <I/σ(I)> | 19.3 | 2.9 |
| Completeness [%] | 96.9 | 95.4 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | Magnesium Sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | Magnesium Sulfate, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
