2ASF

Crystal structure of the conserved hypothetical protein Rv2074 from Mycobacterium tuberculosis 1.6 A

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979571, 1.019691, 1.115869
Spacegroup name	P 43 21 2
Unit cell lengths	73.395, 73.395, 44.794
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.240 - 1.600
R-factor	0.17992
Rwork	0.179
R-free	0.20397
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.271
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.240	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	16283
Completeness [%]	97.6	87.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	20% PEG 4000, 0.2M sodium citrate, 3% dioxane, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K