2AS9
Functional and structural characterization of Spl proteases from staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-05-20 |
| Detector | MACSCIENCE DIP100S |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.120, 81.400, 107.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.210 |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.550 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 51998 | |
| Completeness [%] | 99.9 | 43.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 280 | PEG 8000, Zn-acetate, Na-cacodylate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
