2AS0
Crystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.513, 86.161, 76.693 |
| Unit cell angles | 90.00, 114.16, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 142280 | |
| <I/σ(I)> | 23 | 2.5 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.2 | 298 | 1.6 M Ammonium Sulfate, 16 % Glycerol, 0.1 M Sodium Acetate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
