2ARQ
Human plasminogen activator inhibitor-2.[loop (66-98) deletion mutant] complexed with peptide n-acetyl-teaaagdggvmtgr-oh
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-09 |
Detector | MAC Science DIP-2030 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.562, 104.126, 41.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.800 - 1.850 |
R-factor | 0.18469 |
Rwork | 0.182 |
R-free | 0.24822 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jrr |
RMSD bond length | 0.020 |
RMSD bond angle | 1.708 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.800 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.069 | 0.417 |
Number of reflections | 33539 | |
<I/σ(I)> | 15.2 | 2.1 |
Completeness [%] | 96.2 | 98.1 |
Redundancy | 3.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 8K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |