2ARK
Structure of a flavodoxin from Aquifex aeolicus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2005-05-06 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97911, 0.97924 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 112.846, 112.846, 150.648 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.000 - 2.400 |
R-factor | 0.17396 |
Rwork | 0.171 |
R-free | 0.23519 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.717 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 43827 | |
<I/σ(I)> | 1.8 | |
Completeness [%] | 99.6 | 98.8 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Na-HEPES, Na Citrate, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |