2ARJ

CD8alpha-alpha in complex with YTS 105.18 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-31
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97960
Spacegroup name	P 21 21 21
Unit cell lengths	83.344, 107.135, 131.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.880
R-factor	0.22326
Rwork	0.220
R-free	0.27709
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1ACY for YTS 105.18 Fab PDB Entry: 1BQH for CD8 component
RMSD bond length	0.017
RMSD bond angle	1.684
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.629	3.000
High resolution limit [Å]	2.880	2.884
Rmerge	0.154	0.690
Number of reflections	26797
<I/σ(I)>	10.8	1775
Completeness [%]	99.1	95.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.1	299	20% PEG 3,000, 0.1M Sodium Acetate, pH 5.1, VAPOR DIFFUSION, temperature 299K