2ARH
Crystal Structure of a Protein of Unknown Function AQ1966 from Aquifex aeolicus VF5
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97940, 0.97954 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.611, 121.611, 102.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.460 |
| R-factor | 0.22943 |
| Rwork | 0.226 |
| R-free | 0.29006 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.089 | 0.692 |
| Number of reflections | 28687 | |
| <I/σ(I)> | 2.25 | |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 7.4 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | ammonium sulfate, citric acid, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
