2AQW

Structure of putative orotidine-monophosphate-decarboxylase from Plasmodium yoelii (PY01515)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9797
Spacegroup name	P 61 2 2
Unit cell lengths	61.632, 61.632, 274.284
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.21217
Rwork	0.211
R-free	0.26154
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	1.673
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.900	0.546
Number of reflections	21895
<I/σ(I)>	9.1	2.35
Completeness [%]	99.1	97.1
Redundancy	6	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	297	2.5 M (NH4)2SO4, Bis-Tris propane pH 7.0 and 10mM NaI, VAPOR DIFFUSION, temperature 297K
1	VAPOR DIFFUSION	7	297	2.5 M (NH4)2SO4, Bis-Tris propane pH 7.0 and 10mM NaI, VAPOR DIFFUSION, temperature 297K
1	VAPOR DIFFUSION	7	297	2.5 M (NH4)2SO4, Bis-Tris propane pH 7.0 and 10mM NaI, VAPOR DIFFUSION, temperature 297K