2AQV
Crystal Structure of E. coli Isoaspartyl Dipeptidase mutant Y137F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-09-06 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 119.100, 119.100, 138.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1onw |
| Data scaling software | XDS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 71537 | |
| <I/σ(I)> | 11.6 | 2.5 |
| Completeness [%] | 97.9 | 81.8 |
| Redundancy | 3.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | PEG8000, homopipes, madnesium chloride, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
