2AQT
CU/ZN superoxide dismutase from neisseria meningitidis K91Q, K94Q double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 96 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.209, 64.464, 85.051 |
| Unit cell angles | 90.00, 128.33, 90.00 |
Refinement procedure
| Resolution | 49.440 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild-type |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 38514 | |
| <I/σ(I)> | 11.1 | 2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | AmSO4, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
