2AQQ
CU/ZN superoxid dismutate from neisseria meningitidis K91E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 2001-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.661, 64.685, 82.843 |
| Unit cell angles | 90.00, 125.91, 90.00 |
Refinement procedure
| Resolution | 29.140 - 1.650 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wild-type |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 51225 | |
| <I/σ(I)> | 24.2 | 3.4 |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | AmSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
