2AQO
Crystal structure of E. coli Isoaspartyl Dipeptidase Mutant E77Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-08-20 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 119.200, 119.200, 138.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.950 |
| R-factor | 0.18 |
| Rwork | 0.179 |
| R-free | 0.21900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1onw |
| Data scaling software | XDS |
| Phasing software | TNT |
| Refinement software | TNT (V. 5-E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.040 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 68348 | |
| <I/σ(I)> | 8.2 | 1.7 |
| Completeness [%] | 93.4 | 75.7 |
| Redundancy | 3.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | PEG-8000, MgCl2, homopipes, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
